- Obstetricians & Gynecologists, Middleton - Ob-Gyn Doctors, Physicians & Surgeons (Obstetrics & Gynecology)
- Occupational Health Care Professionals, Middleton
- Occupational Therapy, Middleton - Occupational Health Specialists
- Office Supplies, Equipment & Office Furniture Stores, Middleton
- Oil & Gas Companies, Middleton
- Oil Change & Lubrication, Middleton - Auto Oil & Quick Lube
- Ophthalmologists, Middleton - Eye Doctors, Physicians & Surgeons (Ophthalmology)
- Optical Stores - Eyeglasses, Lenses & Sunglasses, Middleton
- Opticians, Middleton
- Optometrists, Middleton - Eye Doctors, Physicians & Surgeons (Optometry)
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- Organizing Consultants, Middleton
- Oriental Restaurants, Middleton
- Ornamental Nursery, Middleton
- Ornamental Nursery Services, Middleton
- Orthodontist, Middleton - Teeth Straightening, Invisalign & Dental Braces
- Other Building Materials & Supplies, Middleton
- Other Specialties, Middleton - Doctors, Physicians & Surgeons
- Outdoor & Lawn & Garden Equipment Repair & Service, Middleton
- Outdoor Equipment & Accessories, Middleton
- Outdoor Furniture & Patio Furniture, Middleton - Garden Furniture Stores
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